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Intracellular reactive oxygen species (ROS) levels were assessed post-treatments using the fluorescent probe 2,7-dichlorofluorescin diacetate (DCFH-DA)

Distinct from traditional forms of cell death such as apoptosis, necroptosis, autophagy, and pyroptosis, the fundamental mechanism of ferroptosis involves iron overload, which leads to dysregulated lipid peroxidation and excessive accumulation of reactive oxygen species (ROS)

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Sulfur-rich foods : Sulfur-rich foods, such as meat, poultry, fish, eggs, and dairy products, are needed for the body to produce glutathione

The dual chamber device may further comprise passage means allowing passage of liquid from the proximal chamber to the distal chamber past and/or through the first elastomeric stopper