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Rocha-Rodrigues et al., 2016

Detection of Cellular ROS Levels Intracellular and mitochondrial ROS levels were detected using fluorescent measurement assay by the uptake of 1 M DCFH-DA (Beyotime, China) and 5 M MitoSOX Red (Thermo Scientific, USA), respectively

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doi: 10.1053/j.gastro.2023.02.022 77 LiangYShiXShenYHuangZWangJShaoCet al

151,152 The mechanism of IAPP-induced cell death may also include inflammation and oxidative stress

We hypothesize that after delivery into the biofilm-infected sites, SeC@PA can not only deplete GSH and generate reactive nitrogen species (RNS) to enhance the anti-biofilms effect of PDT, but also utilize the high levels of GSH in biofilms to generate hydroxyl radical (OH) through a series of catalytic cascade reactions (Fig