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Conclusion: The present study demonstrated that circulating IGF-1 may be causally related to lower risk of asthma

Secondary antibodies: Fluorescein donkey anti-rat IgG (Jackson ImmunoResearch, Cat#712-095-150, 1:5000), Fluorescein donkey anti-mouse IgG (Jackson ImmunoResearch, Cat#715-095-150, 1:5000), Anti-mouse IgG, HRP-linked (Cell Signaling Technology, Cat#7076, 1:5000), Anti-Rabbit IgG, HRP-linked (Cell Signaling Technology, 7074, 1:5000), Normal mouse IgG (Santa Cruz Biotechnology, Cat#sc-2025, 2g), Normal rabbit IgG (Cell Signaling Technology, Cat#2729S, 2g), Fluorescein (FITC)-AffiniPure Donkey Anti-Rabbit (Jackson Immuno, cat# 711-095-152, 1:5000), Cy3 Goat Anti-Rabbit (Jackson Immuno, cat# 111-165-003, 1:5000), Cy3-AffiniPure Donkey Anti-Mouse (Jackson Immuno, cat# 715-165-150, 1:5000)

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It significantly enhances Nrf-2 activation and the expression of HO-1, NADPH quinine oxidoreductase (NQO1), and glutamate-cysteine ligase catalytic (119)