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Ultimately, the fields progression hinges on interdisciplinary collaboration to integrate adaptive technologies (e.g., AI-driven real-time monitoring), establish standardized safety protocols, and prioritize patient-centered design

Nelson et al

155,156 However, this method provides only semiquantitive data, and the sensitivity is quite low

Digestion-based DNA methylation analysis Percentage DNA methylation was assessed at CpG sites across CpG islands in CYP2R1, CYP27A1, CYP27B1, CYP24A1, and VDR genes using Qiagen EpiTect II methylation enzyme kits and PCR assays at the concentrations and cycle conditions recommended by the manufacturer