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Because EWcp/UCN1/CART neurons express estrogen receptor beta and estrus cycle-related hormonal fluctuations modulate UCN1 levels, we recruited exclusively male mice in our study 85,86

The densely packed collagen and poor vascular supply significantly hinder the penetration of urate-lowering therapies into the core of the tophus and restrict the infiltration of immune cells such as macrophages

Filho AMC, Gomes NS, Ls DB, Leite IB, Tremblay M, Macdo DS

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Extracted proteins were mixed with 3 blue loading buffer pack (Cell Signaling) supplemented with 42 mM DTT, and samples were heated at 95 C for 5 min