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The evidence base is almost entirely preclinical: rodent models consistently show accelerated tissue repair, gut healing, and neurological protection, but human RCT data remains largely absent

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The results revealed statistically significant mean differences in C-reactive protein serum levels between groups at 24 h postadjunctive therapy with melatonin and a significant improvement of clinical condition in neonates from the intervention group compared to the control group within 3 days of therapy 109

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Mouse infection assays To determine the bacterial load in tissues, 24 male Balb/c mice (6 weeks old) were intraperitoneally infected with 200 L of bacterial suspension (1 10 9 CFU/mL), which resulted in 100% mortality at 168 h post infection, and then randomly divided into four groups