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2.4 Histological preparation Extracted ovaries were fixed in 10% NBF for 24 h, then all samples were dehydrated and embedded in paraffin wax blocks, after which they were sectioned (4 m thick) by using the Rotary Microtome, RM 2245 (Leica, Germany) and stained with hematoxylin and eosin (H&E) for classic histological study according to the Mayer modified staining method (Bancroft & Gamble 2008) by using the Autostainer machine (Leica ST 5020, Lecia, Germany)

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152 Campomanesia phaea essential oil gives a marked decrease in the production of IL-6, TNF-, NO, superoxide radical, and NF-B activity when assayed for anti-inflammatory activity

Aging Cell 17 , e12731 (2018)